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Nanostructure by in vivo molecular mechanical chaperonin GroEL

  • Tokyo Universitya・Tokyo Institute of Technologyb・RIKENc
  • Daiki Kashiwagia・Hao Shena・Tatsuya Niwab・Hideki Taguchib・Takuzo Aidaa, c
  • Supporter: Ayumi Kimura (Nanotechnology Platform Advanced Professional Engineer)

　Janus GroEL (AGroELB) was successfully synthesized and isolated. As an application of Janus GroEL to supramolecular copolymerization, we attempted ternary supramolecular copolymerization with two different DNA comonomers.Structural analysis of the supramolecular assembly and visualization of comonomer arrangement with double periodicity Organic materials High-contrast transmission electron microscope JEM-1400 (JEOL) and cryo-transmission electron microscope JEM-2100F (JEOL) (Figures 1 and 2b)
The results were published in Daiki Kashiwagi et al., Journal of the American Chemical Society, 142, 13310 (2020), and were awarded as "outstanding utilization results" in FY3.

Nanostructure by in vivo molecular mechanical chaperonin GroEL
Figure 1. (a) AGroELBand DNA comonomers A* and B* (b).AGroELBCryo-transmission electron micrograph of a copolymer mixture of
Nanostructure by in vivo molecular mechanical chaperonin GroEL
Figure 2. (a)AGroELBand DNA comonomers A** and B*. (b) Transmission electron micrograph of the copolymer mixture.